Coding

Part:BBa_K3867007:Design

Designed by: Lei Huang   Group: iGEM21_ICJFLS   (2021-10-13)


GBSS1 (Granule-bound starch synthase1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
    Illegal BglII site found at 202
    Illegal BamHI site found at 538
    Illegal BamHI site found at 1599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1465


Design Notes

Two enzymes EcoRI and PstI are used to insert the gene into the vector. So these two enzymes are avoided.


Source

This gene comes from Arabidopsis thaliana.

References